Diagnostic device > Antifungal susceptiblity discs
ANTIMYCOTIC SUSCEPTIBILITY DISCS 50 ps Code: DD 601 - DD 613
100 ps DD 6011-DD 6131
DD 601 NYSTATIN (NYS) 50mg
DD 602 AMPHOTERICIN B (AMB) 50mg
DD 603 PIMARICIN (PIM) 50mg
DD 604 5-FLUOROCYTOSIN (5FC) 5mg
DD 605 ITRACONAZOLE (ITR) 30mg
DD 606 KETOCONAZOLE (KET) 30mg
DD 607 CLOTRIMAZOLE (KLO) 30mg
DD 608 MICONAZOLE (MIK) 30mg
DD 609 FLUCONAZOLE (FLU) 25mg
DD 610 ECONAZOLE (EKO) 30mg
DD 611 BIFONAZOLE (BIF) 30mg
DD 612 CIKLOPIROXOLAMIN (CIK) 30mg
DD 613 VORICONAZOLE (VOR) 1mg
Purpose and usage:
Antimycotic susceptibility discs are used for the determination of the susceptibility of microscopic fungi to antimycotic agents in vitro. It is possible to get the information about actual susceptibility of the tested fungal isolate by a simple diffusion method after 18-24 hrs already. The results of the antimycotic antibiogram make it possible to start a rational treatment of the mycotic infection.
Technique:
Prepare a suspension (degree No. 0.5 of McFarland turbidity scale) of the tested strain in saline and flood the surface of an agar plate with this suspension. After suctioning off the remaining liquid and drying the plate (about 15 min. at room temperature) put antimycotic discs on the surface of the plate using aseptic technique. Incubate 18-24 hrs at 37°C.
Evaluation:
After the incubation, observe the presence and eventually the size of the inhibition zones around the discs.
A clear, sharply bordered inhibition zone usually of a small diameter (AMB and PIM > 10 mm, NYS > 15 mm) is characteristic for polyene antibiotics and CIK applied to in vitro susceptible strains.
An inhibition zone with fuzzy margins and visible growth of the tested strain inside the zone is characteristic for azoles (KLO, EKO, BIF, KET, ITR and FLU) and 5FC even in vitro susceptible strains. The size of the inhibition zone is as follows:
5FC, KLO, MIK, EKO, KET > 20 mm
BIF, ITR, CIK > 10 mm
FLU > 15 mm
Note: We recomend modified Sabouraud glucose agar for testing (2% glucose, 1% peptone, 1.5% agar, pH not adjusted). Agar with casitone can be used for azole chemotherapeutics - but not for 5FC - (1.9% Casitone Difco, 1% Yeast Extract Difco, 2% glucose, 1% sodium citrate, 0.1% sodium hydrogen phosphate, 2.5% agar, pH 6.6).
Warning:
1. ANTIMYCOTIC SUSCEPTIBILITY DISCS are designed for laboratory use only.
2. Discs should be stored in a dark place at +4°C and should not be used beyond the expiration date shown on the packing.
