Sodium deoxycholate

AUXILIARY DIAGNOSTIC TESTS 3 ml Cat. No. PT 102 bile solubility test (S. pneumoniae)

Product categories

Purpose and use:

The SODIUM DEOXYCHOLATE test is designed to quickly and easily differentiate Streptococcus pneumoniae from viridans streptococci.

Principle:

The test involves the solubility of S. pneumoniae colonies in sodium deoxycholate. In contrast, colonies of viridans streptococci are not altered by sodium deoxycholate.

Procedure:

There are two modifications of the test:

Resuspend the tested strain in 0.5 ml of saline (turbidity value of 1–2 on McFarland scale) and add 3 drops of sodium deoxycholate. Incubate for 30 min. at 37 °C.
Dispense 1 drop of sodium deoxycholate on the tested strain grown on blood agar and incubate for 30 min at laboratory temperature.

NOTE: Use a suspension of the tested strain in saline without sodium deoxycholate as a control.

Reading the results and evaluation:

After incubation:

Modification 1: Clearing occurs with pneumococci. In case of viridans streptococci and controls, the suspension remains unchanged and turbid.
Modification 2: Within 30 minutes after the tested strain is exposed to sodium deoxycholate, the colonies of pneumococci dissolve compared to those of viridans streptococci, which remain unchanged.

Notice:

The SODIUM DEOXYCHOLATE test is supplied in doses of 3 ml (Cat. No. PT 102) and is for in vitro use only.
Store sodium deoxycholate at laboratory temperature and use by the expiry date on the label.
If the primary packaging of the IVD is damaged, do not use and contact the manufacturer.
Dispose of unused IVDs and empty packaging in accordance with the Waste Act No. 185/2001 Coll.

References

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Teleflex

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