ITEST S. AUREUS LATEX

Cat. num. SL 141

Product categories

Purpose and use

The ITEST S. AUREUS LATEX kit is designed for rapid, easy and accurate identification of Staphylococcus aureus. The starting material for the identification is colonies from blood agar.

Principle

Pathogenic strains of S. aureus produce an enzyme called clumping factor and the cells of these strains contain protein A. Rabbit immunoglobulin (IgG) and fibrinogen are bound to the latex particles of ITEST S. AUREUS LATEX. The reaction of clumping factor with fibrinogen or protein A with IgG results in clear agglutination of the latex suspension. Clumping factor and protein A are mutually independent factors and their detection is important in the identification of S. aureus.

Procedure

Apply one drop of ANTI S. AUREUS latex suspension to the black field of the detection card.
Using the stirring rod, pick up about 5 colonies of the tested strain and mix them well in the latex suspension.
Rock the detection card for one minute. When testing multiple strains at the same time, make sure that material from adjacent areas does not mix. Repeat the test if this happens.

Evaluation

If positive (reaction of clumping factor with fibrinogen or protein A with rabbit IgG), a clear agglutination of the ANTI S. AUREUS latex suspension occurs within one minute.
Agglutination = formation of clumped particles visible to the naked eye with more or less clear fluid between them.

If negative, agglutination of the latex suspension does not occur within one minute.

Note: Weak traces of granular material, which may be observed even in negative cases, should be disregarded.

Important

The ITEST S. AUREUS LATEX kit is supplied in packs with 50 tests (Cat. No. SL 141) and is for in vitro use only.
Keep the kit in a dark place at 2–8 °C and use by the expiry date on the packaging. Latex suspensions must not be allowed to freeze!
When working with reagents, take care to avoid contact with skin and eyes. If contaminated, wash the affected areas immediately with running water.
If the primary packaging of the IVD is damaged, do not use and contact the manufacturer.
Dispose of unused IVDs and empty packaging in accordance with the Waste Act No. 185/2001 Coll.

References

"ITEST is regularly audited by BIOPHARM without significant or critical findings, and is regularly ranked in the highest category in supplier evaluations. Exemplary long-term cooperation provides us with a guarantee of reliability and trust in the quality of services provided."

BIOPHARM

"We started our cooperation with Itest Plus s.r.o at the very beginning of its operation. For almost 30 years, we have been using laboratory services for our own purposes or for contractual testing of our customers' products. We greatly appreciate the professionalism, flexibility and reliability of the entire laboratory team. We would like to thank you for your cooperation so far and look forward to the future."

SVUS Pharma a.s.

"Ferring-Léčiva, a.s. is a company producing sterile and non-sterile medicinal products. We have been cooperating with the ITEST Plus, s.r.o. laboratory for more than 25 years, this cooperation is very valuable to us and we are very satisfied with the range of services and their implementation. The ITEST Plus company offers a wide portfolio of accredited tests, its employees are kind and helpful and respond quickly to all suggestions."

Ferring-Léčiva, a.s.

"We have been cooperating with ITEST plus s.r.o. for many years and are very satisfied with the service, quality of services and communication."

Cayman Pharma

"Itest Plus s.r.o. is for us a synonym for excellent cooperation in the field of laboratory analysis. Professional approach, excellent communication, reliability, speed and accuracy of tests are the calling card of this company and for us the main reasons why this company has been our trusted partner for more than 26 years."

Teleflex

"ITEST provides BIOPHARM with output control of the final product, intermediate products, input raw materials, packaging materials and microbiological monitoring of production areas. The tests are carried out within the scope of sterility and microbial safety tests in accordance with Ph.Eur. and in accordance with the valid contract."

BIOPHARM