ITEST X- and V-FACTOR

DIAGNOSTIC DISCS 100 discs Code: DD 512,513,514
1000 discs DD 5121, 5131, 5141
ITEST X-FACTOR
ITEST V-FACTOR
ITEST X+V-FACTOR
for the differentiation of Haemophili

Product categories

Purpose and use

The ITEST X-FACTOR, V-FACTOR and X+V-FAKTOR diagnostic discs are designed for easy differentiation of Haemophilus species.

Principle

The test is based on the different needs of different types of haemophilia for growth factors X (hemin) and V (NAD).

Procedure

Inoculate blood-free agar medium (Mueller Hinton Agar or Columbia Blood Agar Base) evenly with the tested strain. Apply sterile growth factor diagnostic discs and incubate at 37 ± 1 °C for 24 (or 48) hours.

Evaluation

After incubation, observe the colonies growing around the discs:

bacteria requiring growth factor X grow only in the vicinity of the X and X+V discs
bacteria requiring growth factor V grow only in the vicinity of the V and X+V discs
bacteria requiring both growth factors grow only around the X+V discs

Type	X-FAKTOR	V-FAKTOR
H. influenzae	+	+
H. aegyptius	+	+
H. haemolyticus	+	+
H. ducreyi	+	-
H. haemoglobinophilus	+	-
H. parainfluenzae	-	+
H. parahaemolyticus	-	+
H. paraphrophilus	-	+
H. segnis	-	+
H. aphrophilus	-	-

Recommendations

The following haemophilia strains are recommended for quality control of diagnostic factor discs:

Strain	Required factor
Haemophilus influenzae CCM 4457	X+V
H. haemoglobinophilus CCM 4648	X
H. segnis CCM 6052	V
H. aphrophilus CCM 3582	Žádný

All strains are available from the Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno.

Important

ITEST X-FACTOR, ITEST V-FACTOR and ITEST X+V-FACTOR discs are supplied in packs of 100 (Cat. No. DD 512, DD 513 and DD 514) or 1000 (Cat. No. DD 5121, DD 5131 and DD 5141) and are intended for in vitro use only.
Keep ITEST X-FAKTOR discs in a dark place at 2–8 °C, and ITEST V-FAKTOR and ITEST X+V FAKTOR discs in a dark place at -18 °C. Use the discs by the expiry date on the label.
If the primary packaging of the IVD is damaged, do not use and contact the manufacturer.
Dispose of unused IVDs and empty packaging in accordance with the Waste Act No. 185/2001 Coll.

References

"ITEST is regularly audited by BIOPHARM without significant or critical findings, and is regularly ranked in the highest category in supplier evaluations. Exemplary long-term cooperation provides us with a guarantee of reliability and trust in the quality of services provided."

BIOPHARM

"We started our cooperation with Itest Plus s.r.o at the very beginning of its operation. For almost 30 years, we have been using laboratory services for our own purposes or for contractual testing of our customers' products. We greatly appreciate the professionalism, flexibility and reliability of the entire laboratory team. We would like to thank you for your cooperation so far and look forward to the future."

SVUS Pharma a.s.

"Ferring-Léčiva, a.s. is a company producing sterile and non-sterile medicinal products. We have been cooperating with the ITEST Plus, s.r.o. laboratory for more than 25 years, this cooperation is very valuable to us and we are very satisfied with the range of services and their implementation. The ITEST Plus company offers a wide portfolio of accredited tests, its employees are kind and helpful and respond quickly to all suggestions."

Ferring-Léčiva, a.s.

"We have been cooperating with ITEST plus s.r.o. for many years and are very satisfied with the service, quality of services and communication."

Cayman Pharma

"Itest Plus s.r.o. is for us a synonym for excellent cooperation in the field of laboratory analysis. Professional approach, excellent communication, reliability, speed and accuracy of tests are the calling card of this company and for us the main reasons why this company has been our trusted partner for more than 26 years."

Teleflex

"ITEST provides BIOPHARM with output control of the final product, intermediate products, input raw materials, packaging materials and microbiological monitoring of production areas. The tests are carried out within the scope of sterility and microbial safety tests in accordance with Ph.Eur. and in accordance with the valid contract."

BIOPHARM