Itest Cryobank B

for bacteria (PP 64, PP 641)
ITEST CRYOBANK K for yeast (PP 65, PP 651)

DIAGNOSTIC AIDS 5 pcs Cat. No. PP 64, PP 65
25 pcs Cat. No. PP 641, PP 651

Product categories

Purpose and use:

Kits ITEST CRYOBANK B a ITEST CRYOBANK K are sterile cryotubes with porous beads and medium. They are designed for long-term preservation of bacteria and yeasts at low temperatures (deep-freeze cabinets, liquid nitrogen).

Procedure:

Use a sterile bacteriological loop to take an 18–24 hours old culture of the microorganism and resuspend it in 1 cryotube with porous beads and medium (turbidity value of 3–4 on the McFarland scale).
Mix the cryotube well (by shaking gently) and then aspirate the suspension so that there is no medium above the beads.
Close the cryotube tightly, label it and place it in a freeze cabinet or liquid nitrogen container.
You can use the remainder of the aspirated suspension for inoculation as a check on the purity of the microbial strain.

Cryotube inoculation:

Remove one bead from the cryotube sterilely (with forceps or needle) and place it in a suitable liquid medium or on solid medium (in this case, drop liquid medium over the bead). Then cultivate the medium as usual.
After removing the bead, immediately close the cryotube and place it back into the freeze cabinet or liquid nitrogen.

Note:

Viability period of preserved strains:

at least half a year at -20 °C
at least one year at -40 °C
several years at -70 °C and lower

Notice:

ITEST CRYOBANK B and ITEST CRYOBANK K jare supplied in packs of 5 cryotubes or 25 tubes in a special stand and are intended for in vitro use only. Each cryotube contains 30 porous beads and growth medium.
Keep the kits in a dark place at 2–8 °C and use by the expiry date on the label.
If the primary packaging of the IVD is damaged, do not use and contact the manufacturer.
Dispose of unused IVDs and empty packaging in accordance with the Waste Act No. 185/2001 Coll.

References

"ITEST is regularly audited by BIOPHARM without significant or critical findings, and is regularly ranked in the highest category in supplier evaluations. Exemplary long-term cooperation provides us with a guarantee of reliability and trust in the quality of services provided."

BIOPHARM

"We started our cooperation with Itest Plus s.r.o at the very beginning of its operation. For almost 30 years, we have been using laboratory services for our own purposes or for contractual testing of our customers' products. We greatly appreciate the professionalism, flexibility and reliability of the entire laboratory team. We would like to thank you for your cooperation so far and look forward to the future."

SVUS Pharma a.s.

"Ferring-Léčiva, a.s. is a company producing sterile and non-sterile medicinal products. We have been cooperating with the ITEST Plus, s.r.o. laboratory for more than 25 years, this cooperation is very valuable to us and we are very satisfied with the range of services and their implementation. The ITEST Plus company offers a wide portfolio of accredited tests, its employees are kind and helpful and respond quickly to all suggestions."

Ferring-Léčiva, a.s.

"We have been cooperating with ITEST plus s.r.o. for many years and are very satisfied with the service, quality of services and communication."

Cayman Pharma

"Itest Plus s.r.o. is for us a synonym for excellent cooperation in the field of laboratory analysis. Professional approach, excellent communication, reliability, speed and accuracy of tests are the calling card of this company and for us the main reasons why this company has been our trusted partner for more than 26 years."

Teleflex

"ITEST provides BIOPHARM with output control of the final product, intermediate products, input raw materials, packaging materials and microbiological monitoring of production areas. The tests are carried out within the scope of sterility and microbial safety tests in accordance with Ph.Eur. and in accordance with the valid contract."

BIOPHARM