Diagnostic device > Diagnostic discs
DIAGNOSTIC DISCS 50 discs Code: DD 505
ITEST OXIDASE
for rapid detection of the oxidase activity
Purpose and usage:
OXIDASE diagnostic discs are designed for the rapid presumptive detection of cytochrome oxidase. The test differentiates e.g. pseudomonads, aeromonads etc. from gram negative enteric rods of the family Enterobacteriaceae.
Principle:
The principle is the detection of the cytochrome oxidase by the color reaction of ethyloxethylparaphenylenediamine with alpha-naphtol during which indophenol blue develops.
Technique:
Put the tested culture on the surface of the disc in shape of a line. Read the color reaction during 0.5 - 1 min.
Evaluation:
If the reaction is positive (Oxidase +) the inocula- ted line on the disc turns into deep blue to black. If the reaction is negative (Oxidase -) the inoculated line will stay colorless.
Warning:
1. OXIDASE discs are designed for laboratory use only.
2. Discs should be stored in a dark place at +4oC and should not be used beyond the expiration date shown on the packing.
