Diagnostic device > Diagnostic agglutination antisera
ANTISERA FOR SLIDE AGGLUTINATION Code: AS 415
ESCHERICHIA COLI O:157 H7
Purpose and usage:
Antiserum ESCHERICHIA COLI O:157 H7 is designed for the rapid and easy determination of serological group of E. coli O:157 H7.
Technique:
1. Put one drop of the steril saline on a slide and resuspend in it one colony of the tested strain of E. coli (use 24hours culture).
2. Next to this drop put one drop of the antiserum and mix well both drops.
3. Rock the slide for 1 minute by circling movements.
Reading the results and evaluation of the reaction:
Perform the reading against dark background:
- forming of agglutinates and clearing the surrounding liquid: + positive reaction
- agglutinates do not form and the liquid remains turbid: - negative reaction
Evaluation of the results:
1. Positive reaction – tested strain of E. coli is serologic group O:157 H7
2. Negative reaction – tested strain of E. coli is from other serological group
NOTE: If the agglutination of tested strain in saline occurs, the tested strain is spontaneously agglutinating and it is unsuitable for slide agglutination testing.
Warning:
1. Antiserum is protected with sodium azide. Nevertheless it is necessary to avoid its contamination during its use (you must not touch the slide with the dropper during dropping the serum!).
2. Antiserum is delivered in liquid form 1 ml or 2 ml and is designed for laboratory use only.
3. Antiserum should be kept in a dark place at +4°C and should not be used beyond the expiration date shown on the packing.
