Itest Tributyrin

50 discs Cat. No. DD 504 test for the detection of tributyrin hydrolysis

Product categories

Purpose and use

ITEST TRIBUTYRIN diagnostic discs are designed for the detection of tributyrin hydrolysis. This test allows easy differentiation of some bacterial species, e.g. Branhamella from Neisseria.

Principle

Tributyrin hydrolysis is reflected by a change in the colour of the indicator.

Procedure

Prepare a thick suspension of the tested strain in 1 ml of buffered saline (pH 7.2) (turbidity value of 3 on McFarland scale) and add 1 ITEST TRIBUTYRIN disc. Incubate at 37 ± 1 °C. The colour reaction will begin to appear after a few hours; make a final evaluation after about 20 hours.

Evaluation

If positive (Tributyrin+), the suspension turns yellow; if negative (Tributyrin-) the colour of the suspension remains red.

Recommendations

It is recommended to use Moraxella (B.) catarrhalis CCM 4391 strain as a positive control and Neisseria subflava CCM 3482 strain as a negative control for quality control of ITEST ONPG diagnostic discs. Both strains are available from the Czech Collection of Microorganisms, Masaryk University, Tvrdého 14, 602 00 Brno.

Important

ITEST TRIBUTYRIN discs are supplied in packs of 50 (Cat. No. DD 504) and are intended for in vitro use only.
Keep the discs in a dark place at 2–8 °C and use by the expiry date on the label.
If the primary packaging of the IVD is damaged, do not use and contact the manufacturer.
Dispose of unused IVDs and empty packaging in accordance with the Waste Act No. 185/2001 Coll.

References

"ITEST is regularly audited by BIOPHARM without significant or critical findings, and is regularly ranked in the highest category in supplier evaluations. Exemplary long-term cooperation provides us with a guarantee of reliability and trust in the quality of services provided."

BIOPHARM

"We started our cooperation with Itest Plus s.r.o at the very beginning of its operation. For almost 30 years, we have been using laboratory services for our own purposes or for contractual testing of our customers' products. We greatly appreciate the professionalism, flexibility and reliability of the entire laboratory team. We would like to thank you for your cooperation so far and look forward to the future."

SVUS Pharma a.s.

"Ferring-Léčiva, a.s. is a company producing sterile and non-sterile medicinal products. We have been cooperating with the ITEST Plus, s.r.o. laboratory for more than 25 years, this cooperation is very valuable to us and we are very satisfied with the range of services and their implementation. The ITEST Plus company offers a wide portfolio of accredited tests, its employees are kind and helpful and respond quickly to all suggestions."

Ferring-Léčiva, a.s.

"We have been cooperating with ITEST plus s.r.o. for many years and are very satisfied with the service, quality of services and communication."

Cayman Pharma

"Itest Plus s.r.o. is for us a synonym for excellent cooperation in the field of laboratory analysis. Professional approach, excellent communication, reliability, speed and accuracy of tests are the calling card of this company and for us the main reasons why this company has been our trusted partner for more than 26 years."

Teleflex

"ITEST provides BIOPHARM with output control of the final product, intermediate products, input raw materials, packaging materials and microbiological monitoring of production areas. The tests are carried out within the scope of sterility and microbial safety tests in accordance with Ph.Eur. and in accordance with the valid contract."

BIOPHARM