NEISSERIA MENINGITIDIS type A, C, X, Y

NEISSERIA MENINGITIDIS type A, C, X, Y ANTISERA FOR SLIDE AGGLUTINATION Cat. No. AS 401 – AS 405 NEISSERIA MENINGITIDIS type A AS 401 NEISSERIA MENINGITIDIS type C AS 403 NEISSERIA MENINGITIDIS type X AS 404 NEISSERIA MENINGITIDIS type Y AS 405

Product categories

Purpose and use

The antisera are designed to determine the serotypes of Neisseria meningitidis of groups A, B, C, X and Y. These groups include over 90% of N. meningitidis serotypes isolated from patients with severe meningococcal disease (meningitis, sepsis).

Reaction

Dispense one drop each of antiserum A, B, C, X and Y and a drop of saline solution onto a slide.
Resuspend a colony of the tested Neisseria meningitidis strain in each drop of antiserum (saline) (use 16–18 hours old cultures for max. 3rd passage).
Rock the slide in a circular motion for 1–3 minutes.

Reading the results and evaluating the reaction

Take readings against a dark background:

formation of large agglutinates with distinctive clearing of surrounding fluid: +++
formation of small clots with slightly turbid surrounding fluid: ++
formation of very small bordered or thread-like agglutinates with turbid surrounding fluid: +
no agglutinate formation and the suspension remains unchanged and turbid: -

Evaluation

1. Positive reaction with one antiserum: N. meningitidis type A, B, C, X or Y.
2. Positive reactions with multiple antisera: Polyagglutinable (PA) N. meningitidis.
3. Negative reactions with all antisera: Spontaneously agglutinating (SA) N. meningitidis.
4. Negative reactions with all antisera and saline: Non-agglutinating (NA) N. meningitidis – necessary to indicate that antisera A, B, C, X and Y were used.

Recommendations

The following control strains are recommended for quality control of diagnostic antisera:

N. meningitidis CCM 4634 – serotype A
N. meningitidis CCM 4635 – serotype B
N. meningitidis CCM 4636 – serotype C
N. meningitidis CCM 4637 – serotype X
N. meningitidis CCM 4638 – serotype Y

All strains are available from the Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno.

Important

Antisera are supplied in liquid doses of 1 or 2 ml and are intended for in vitro use only.
Keep the antisera in a dark place at 2–8 °C and use by the expiry date on the package.
If the primary packaging of the IVD is damaged, do not use and contact the manufacturer.
Dispose of unused IVDs and empty packaging in accordance with the Waste Act No. 185/2001 Coll.

Note: Meningococcal strain – interest in sending all strains of N. meningitidis isolated from patients with severe meningococcal disease, or from their contacts, as well as non-agglutinating strains with antisera A, B, C, X and Y.

References

"ITEST is regularly audited by BIOPHARM without significant or critical findings, and is regularly ranked in the highest category in supplier evaluations. Exemplary long-term cooperation provides us with a guarantee of reliability and trust in the quality of services provided."

BIOPHARM

"We started our cooperation with Itest Plus s.r.o at the very beginning of its operation. For almost 30 years, we have been using laboratory services for our own purposes or for contractual testing of our customers' products. We greatly appreciate the professionalism, flexibility and reliability of the entire laboratory team. We would like to thank you for your cooperation so far and look forward to the future."

SVUS Pharma a.s.

"Ferring-Léčiva, a.s. is a company producing sterile and non-sterile medicinal products. We have been cooperating with the ITEST Plus, s.r.o. laboratory for more than 25 years, this cooperation is very valuable to us and we are very satisfied with the range of services and their implementation. The ITEST Plus company offers a wide portfolio of accredited tests, its employees are kind and helpful and respond quickly to all suggestions."

Ferring-Léčiva, a.s.

"We have been cooperating with ITEST plus s.r.o. for many years and are very satisfied with the service, quality of services and communication."

Cayman Pharma

"Itest Plus s.r.o. is for us a synonym for excellent cooperation in the field of laboratory analysis. Professional approach, excellent communication, reliability, speed and accuracy of tests are the calling card of this company and for us the main reasons why this company has been our trusted partner for more than 26 years."

Teleflex

"ITEST provides BIOPHARM with output control of the final product, intermediate products, input raw materials, packaging materials and microbiological monitoring of production areas. The tests are carried out within the scope of sterility and microbial safety tests in accordance with Ph.Eur. and in accordance with the valid contract."

BIOPHARM